Fig. 1

ZBD-2-mediated relief of anxiety- and depression-like behaviors in PPD models. a, b OF test showed that total distance traveled and the time in the central area were significantly reduced in the PPD mice as compared with those of the control. ZBD-2 relieved anxiety-like behaviors, as indicated by the increased total distance traveled and the time in the central area. PK11195 abolished the effects of ZBD-2 on total distance traveled and the time in the central area. c, d EPM test showed that the number of open-arm entries and the time spent in open arm were markedly decreased in the PPD models. ZBD-2 treatment reversed the number of open-arm entries and the time spent in open arm in the PPD mice. The effects of ZBD-2 were blocked by PK11195. e-g ZBD-2 obviously increased the sucrose intake in the PPD mice (e). In the FST and TST tests, ZBD-2 decreased immobility time in the PPD mice (f, g). The effects of ZBD-2 were abolished by PK11195 (e-g), and the effects of ZBD-2 (1.5 mg/kg) were comparable to those of fluoxetine in all behavior tests (a-g). n = 6 in each group. **p < 0.01 compared with the control; ^#p < 0.05, ^##p < 0.01 compared with PPD model; ^$p < 0.05, ^$$p < 0.01 compared with the ZBD-2 (1.5 mg/kg) group. PK: PK11195