Fig. 10

Electron microphotographs of the superficial layer of the Vc after triple-labeling with WGA-HRP, FG and CGRP. Axon terminals labeled with CGRP (c, d) form asymmetric synapses (black arrowheads) with WGA-HRP and FG dually labeled dendritic profiles (De) in the superficial layer of the Vc after a WGA-HRP injection into the right thalamus and an FG injection into the left LPB. CGRP-LI is represented with electron-dense, amorphous products of DAB reaction (c, d). Silver-intensified gold particles produced with the immunogold-silver method indicate FG labeling (b, d; arrows). Electron-dense, amorphous material produced by the peroxidase reaction indicate WGA-HRP labeling (TMB reaction; a, d; double arrowheads). De, dendritic profiles; T, CGRP-LI axon terminals; *, CGRP-negative axon terminals. Scale bar 250 nm (a, b, c and d)