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Fig. 10 | Molecular Brain

Fig. 10

From: VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats

Fig. 10

Electron microphotographs of the superficial layer of the Vc after triple-labeling with WGA-HRP, FG and CGRP. Axon terminals labeled with CGRP (c, d) form asymmetric synapses (black arrowheads) with WGA-HRP and FG dually labeled dendritic profiles (De) in the superficial layer of the Vc after a WGA-HRP injection into the right thalamus and an FG injection into the left LPB. CGRP-LI is represented with electron-dense, amorphous products of DAB reaction (c, d). Silver-intensified gold particles produced with the immunogold-silver method indicate FG labeling (b, d; arrows). Electron-dense, amorphous material produced by the peroxidase reaction indicate WGA-HRP labeling (TMB reaction; a, d; double arrowheads). De, dendritic profiles; T, CGRP-LI axon terminals; *, CGRP-negative axon terminals. Scale bar 250 nm (a, b, c and d)

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Molecular Brain

ISSN: 1756-6606