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Fig. 4 | Molecular Brain

Fig. 4

From: VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats

Fig. 4

Collateral projection neurons to both the thalamus and the PBN labeled with hybridization signals for VGLUT1 mRNA in the Vo (a, a1-a4), Vi (b, b1-b4) and Vc (c, c1-c4). Some neurons are retrogradely labeled with TMR and FG. Immunoreactivity for TMR is visualized with Alexa 594 (red), and immunoreactivity for FG is visualized with Alexa 647 (blue), whereas the VGLUT1 mRNA hybridization signals are shown with fluorescein (green). Images (a1-a4), (b1-b4) and (c1-c4) are the magnified images of the framed areas in (a), (b) and (c), respectively. The arrows in (a1-a4) indicate the cell bodies triply labeled with TMR/FG/VGLUT1 mRNA signals (white); the double arrowheads in (a1-c4) indicate the cell bodies dually labeled with TMR and FG (purple red); the single arrowheads in (a1-c4) point to cell bodies singly labeled with FG (blue). Scale bar 300 μm (a-c); 100 μm (a1-c4)

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