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Fig. 7 | Molecular Brain

Fig. 7

From: VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats

Fig. 7

Vc neurons that send axon collaterals to both the thalamus and PBN are Fos positive after a subcutaneous 4% formalin injection into the left upper lip. TMR was injected into the right VPM and Po of the thalamus, and FG was injected into the left PBN. TMR, FG and Fos immunoreactivity in the Vc are visualized with Alexa 594 (red), Alexa 647 (blue) and fluorescein (green), respectively. a-d Representative section from animals injected with normal saline (R4, R7, and R13, Fig. 2) and showing triple-labeled with TMR, FG and Fos. e-h Representative section from animals injected formalin (R18, R25, and R29, Fig. 6) and showing triple-labeled with TMR, FG and Fos. The double arrowheads in (b, c and d) indicate neurons double-labeled with TMR/FG. The single arrowheads in (a-h) indicate neurons double-labeled with FG and Fos. The arrows in e-h indicate neurons triple-labeled with TMR/FG/Fos. I, II and III in (d) and (h) represent the laminae I, II and III of Vc. Scale bar 50 μm

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