VGLUT1 or VGLUT2 mRNA-positive neurons in spinal trigeminal nucleus provide collateral projections to both the thalamus and the parabrachial nucleus in rats

Table 3 Number of neurons labeled with FG, TMR and (or) Fos in the Vc of R18, R25 and R29

	Lamina I	Lamina II	Lamina III	Total
(1)TMR	92 ± 9 (59.3 ± 1.5)%	12 ± 3 (7.9 ± 2.5)%	51 ± 7 (33.1 ± 0.3)%	142 ± 18
(2)FG	81 ± 8 (57.5 ± 1.8)%	21 ± 4 (15.5 ± 4.0)%	39 ± 6 (27.6 ± 0.7)%	155 ± 18
(3)Fos	344 ± 18 (29.3 ± 0.4)%	287 ± 11 (24.5 ± 1.7)%	541 ± 16 (46.2 ± 0.5)%	1172 ± 46
(4)TMR + FG	29 ± 6 (64.0 ± 4.4)%	7 ± 2 (15.5 ± 6.5)%	11 ± 4 (22.1 ± 2.9)%	47 ± 12
(5)TMR + FG + Fos	13 ± 4 (77.8 ± 2.1)%	2 ± 1 (14.2 ± 7.0)%	2 ± 0 (10.4 ± 1.6)%	16 ± 5
(6)[(5)/(4)] × 100	(42.1 ± 5.8)%	(29.4 ± 3.4)%	(16.4 ± 3.1)%	(34.4 ± 2.4)%

TMR is injected into the collateral thalamus, and FG is injected into the ipsilateral PBN. The cell body counts were performed for three rats on the ipsilateral side of the FG injection site. Each figure showing the number of cell bodies of one animal was the average of the three rats (10 sections from each rat). The sections were 20 μm thick. FG, TMR and Fos represent all FG-, TMR- and Fos-labeled neurons; data are shown as the mean ± standard deviation (SD)

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