Fig. 2
Treg enhance OPC differentiation and reduce neuronal percentages in mixed neuron-glia cultures. Mixed neuron-glia cultures were treated with 5% Treg-conditioned media or matched medium control for 5 days. Immunofluorescence analysis (a) of Olig2+ oligodendroglial cell population for percentage of OPCs (PDGFRα+), oligodendrocytes (MBP+) and proliferating OPCs (Ki67+) and (b) of DAPI+ cells for percentage of oligodendroglial lineage cells (Olig2+), neurons (MAP2+) and microglia (CD11b+), n = 6 wells, mean +/− SEM, Mann-Whitney test, (*) p < 0.05, (**) p < 0.01, representative of two independent experiments. Data are from 5 different experiments depending on timepoint and marker. FOV = field of view = 1 mm2