Fig. 3

Exogenous Treg-polarizing factors do not directly affect OPC differentiation. (a) Mixed neuron-glia cultures were treated with Treg-polarizing media (pol. media), a mixture of TGF-β (300 pg/ml), anti-IFN-γ (500 ng/ml) and IL-2 (500 pg/ml), compared to Treg-conditioned media and matched medium control (X-VIVO™15) for 5 days (b) Pure OPC cultures were treated with a mixture of TGF-β (300 pg/ml), anti-IFN-γ (500 ng/ml) and IL-2 (500 pg/ml) compared to Treg-conditioned media and matched medium control (BSM) for 3 days. Immunofluorescence analysis of MBP⁺ area, n = 6 wells, mean +/− SEM, unpaired t-test, (***) p < 0.001, representative of two independent experiments. FOV = field of view. (c) Representative images of pure OPC cultures stained for oligodendrocytes (Olig2⁺MBP⁺) at 10× and 40× magnification at 12 div