Fig. 1
From: T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B
Identification of spectrin (a/β) as a Cav3.1 / Cav3.2 calcium channel interacting protein. (a) Conserved proximal C-terminus region of Cav3.1 and Cav3.2 calcium channels. (b) Proteins bound to scramble (lane 1) or Cav3.2 CT 1860–1884 biotinylated peptides (lane 2) from mouse whole brain lysates, as seen by Coomassie staining (c) Mass spectrometry analysis of proteins bound to the Cav3.2 CT 1860–1884 biotinylated peptide by affinity precipitation assay using mouse whole brain lysates. (d) Cav3.1 or (e) Cav3.2 immunoprecipitates from mouse whole brain lysates were probed for spectrin αII (SPTAN1) by Western blot. An actin loading control is shown