Fig. 5
From: T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B
Mobility of wild type and mutant Cav3.1 and Cav3.2 channels in hippocampal neurons. (a) Fluorescence recovery after photobleaching (FRAP) assay of Cav3.1-GFP wild type and ∆CT (1851–1875) mutant channels or Cav3.2-GFP wild type and ∆CT (1860–1884) mutant channels transfected into mouse hippocampal neuron cultures. (b) Recovery (Fluorescence-Fluorescence bleach) of Cav3.1-GFP wild type and ∆CT (1851–1875) mutant channels. Recovery values are calculated as F(maximum after photobleach)-F(photobleach) (n = 8 WT, n = 8 mutant, P < 0.01, Student’s t-test). (c) Recovery (Fluorescence-Fluorescence bleach) of Cav3.2-GFP wild type and ∆CT (1860–1884) mutant channels. Recovery values are calculated as F(maximum after photobleach)-F(photobleach) (n = 11 WT, n = 12 mutant, P < 0.01, Student’s t-test)