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Fig. 6 | Molecular Brain

Fig. 6

From: T-type calcium channels functionally interact with spectrin (α/β) and ankyrin B

Fig. 6

SPTAN1 and ankyrin B knockdown effect on endogenous Cav3.2 calcium channel density in mouse and rat hippocampal neurons. (a) SPTAN1 expression levels in mouse hippocampal neurons treated with shRNA as seen by western blot. (b) Mouse hippocampal neurons untreated or treated with shRNA for SPTAN1 were stained for Cav3.2 channels with a specific anti-Cav3.2 polyclonal antibody. (c) Cav3.2 channel intensity values from untreated neurons or neurons treated with SPTAN1 shRNA. (d) Ankyrin B expression levels in rat hippocampal neurons treated with shRNA as seen by western blot. (e) Rat hippocampal neurons untreated or treated with shRNA for ankyrin B were stained for Cav3.2 channels with a specific anti-Cav3.2 polyclonal antibody. (f) Cav3.2 channel intensity values from untreated neurons or neurons treated with specific ankyrin B shRNA

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