Fig. 4

INI-0602 did not prevent HFD-induced locomotor activity pattern disturbances in mice. Locomotor activities were measured in the same mice shown in Fig. 4, for the vehicle group (V, n = 7, white squares with dashed line) and the treatment group (Tx, n = 8, black circles with solid line). White and black bars on the X-axis correspond to the light and dark cycles, respectively. Vertical dashed line indicates the switch from NC (Pre) to HFD and the initiation of IP injections (Post). (a–b) Hourly locomotor activity patterns, measured over the course of the study. Locomotor activity is shown for (a) vehicle (b) or INI-0602 groups. (c–e) Locomotor activities measured during (c) the light cycle, (d) the dark cycle, and (e) each 24-h period. (f–h) Cosinor analyses of the locomotor activity rhythms, including the (f) period length, (g) amplitude, and (h) nadir of the CT. (i) Actograms of two representative mice in each group. White and black bars above the traces correspond to the light and dark cycles, respectively. Data are the means ± s.e.m. Statistical significance was determined with the Student’s t-test, for comparisons between the two groups at each time point, in a–c; *P < 0.05. Abbreviations: NC normal chow, HFD high-fat diet, A.U. arbitrary unit, CT circadian time