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Fig. 3 | Molecular Brain

Fig. 3

From: Excitation wavelength optimization improves photostability of ASAP-family GEVIs

Fig. 3

Improved photostability of ASAP2f with single 440-nm illumination. a Unlike 470-nm illumination, 440-nm light elicited much less photoswitching of ASAP2f in transfected neurons and was not further improved by assistive 405-nm co-illumination (indicated by a purple bar). b Fluorescence response of ASAP2f to single APs remained unchanged under 440-nm illumination (440-nm: - 5.7 ± 0.6%, n = 7; 470-nm: - 6.1 ± 0.8%, n = 6; n.s.: p > 0.05, t-test)

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