Fig. 1

Generation and characterization of NL2 R215H mice. a A simplified diagram of NL2 R215H homologous recombination strategy. b Representative Western blot of NL2 protein expression at postnatal 2 days, 6 days, 21 days, and adult stage of WT, NL2 R215H Het, and NL2 R215H KI mice. GAPDH was used as internal control. c Quantification of NL2 protein expression level in littermates. WT, n = 6 mice, Het, n = 5 mice, and KI, n = 6 mice. One-way ANOVA with post-hoc Tukey multi-comparison test was used for statistical analysis. d Representative images of NL2 postsynaptic puncta in WT, NL2 R215H Het and NL2 R215H KI mice. Images were taken at hippocampal CA1 region. Upper row scale bar = 10 μm. Bottom row scale bar = 5 μm. e, f Quantification of NL2 puncta number and size. Nine brain slices from 3 mice for each genotype were used for analysis. One-way ANOVA with post-hoc Tukey multi-comparison test was used for statistical analysis in (e). Student’s t-test was used for statistical analysis in (f). Data were shown as Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001