Fig. 4

Blockade of DNMT1 acts in synergy with IFNγ to induce MHC-I gene expression. Panel A-C show the effect of combined treatment of N2a cells with IFNγ and 5-aza. Fold change in gene expression of H2-K1 (a), H2-D1 (b), and H2-D1/L (c) relative to untreated N2a cells. Bars represent mean +/− S.E.M., N = 3. Panel D-E show the effect of simultaneous treatment of CGNs with IFNγ and siRNA against DNMT1 or non-targeting (NT) control siRNA. Fold change in gene expression of H2-D1 (d), and H2-D1/L (e) relative to untreated CGNs. Bars represent mean +/− S.E.M., N = 6 from two independent experiments. Panel F-G show the effect of treatment of CGNs with siRNA against DNMT1 for 72 h, followed by treatment with IFNγ for the next 72 h. Fold change in gene expression of H2-D1 (f), and H2-D1/L (g) relative to untreated CGNs. Bars represent mean +/− S.E.M., N = 3. Baselines indicate gene expression level in CGNs treated with non-targeting (NT) siRNA. Stars in A-C illustrate effects that are significantly different from untreated CGNs, and stars in D-G illustrate effects that are significantly different from CGNs treated with non-targeting siRNA, unless otherwise indicated. * p < 0.05, ** p < 0.01, *** p < 0.001, multiplicity corrected p-values in a One-Way ANOVA with Dunnett’s post hoc test