Fig. 1
From: Dysregulation of human NEFM and NEFH mRNA stability by ALS-linked miRNAs
Single NEFM and NEFH mRNA 3’UTR variants are expressed in human spinal cord. 3’RACE-PCR was performed from spinal cord tissue samples of control individuals using specific primers to amplify NEFM and NEFH 3’UTRs. 3’UTRs were cloned and sequenced. One 3’UTR variant for each NEFM (486 nt) and NEFH (583 nt) transcript was observed in human spinal cord regardless of control or ALS origin of tissue. NEFM specific primer anneals to a region 40 nt upstream the stop codon