Fig. 6
From: Dysregulation of human NEFM and NEFH mRNA stability by ALS-linked miRNAs
NEFM and NEFH transcript and protein levels are increased in spinal cord of ALS patients. (a) Real-time PCR using TaqMan and (b) Western blots were performed using ventral lumbar spinal cord samples of controls and ALS patients. (c) Quantification of Western blots in (b). Protein levels were normalized to GAPDH. Data was expressed as the mean ± SEM (Student t-test: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05. Real-time PCR: NEFM, p = 0.0027: NEFH, p = 0.0239. Western blot: NFM, p = 0.0238; NFH, p = 0.4762)