Fig. 2

Na⁺/K⁺-ATPase α1 subunits co-immunoprecipitate multiple myosins expressed in adult rat brain. WT adult rat brain lysates (In, lane 2 in a, b and c) were precleared (PC) with mouse IgG1 antibodies (PC, lane 1 in a, b and c) prior to immunoprecipitation (IP) using mouse anti-Na⁺/K⁺-ATPase α1 antibodies of the IgG1 isotypes (IP, lane 3 in a, b and c). Loading of PC complexes in the gel preceded those of the lysate inputs (In). Na⁺/K⁺-ATPase α1 subunits co-immunoprecipitated myh9 (a (i)), myh10 (b, (i)), myoVa (c (i)), β-actin ((iii) in a, b and c) and myosin regulatory light chain (MRLC) ((iv) in a, b and c) from rat brain. An asterisk (‘*’; (iv) in a, b and c) indicates lack of detection of the input signal for the MRLCs. As expected anti-Na⁺/K⁺-ATPase α1 antibodies immunoprecipitated Na⁺/K⁺-ATPase α1 subunits expressed in brain tissues ((ii) in a, b and c)