Skip to main content

Advertisement

Fig. 4 | Molecular Brain

Fig. 4

From: Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force

Fig. 4

Producing focal dMCA occlusion in perinatal and neonatal brains by SIMPLE. a. Permanent MP occlusion (arrowhead) was formed in the dMCA of a P3 mouse (~ 2 g) after 20 min SIMPLE (MP dose of 50 μg/g). Magnet was removed after 20 min, which is long enough to form permanent MP occlusion. The pup was sacrificed at 24 h after SIMPLE. b. Permanent MP occlusion was formed in the dMCA of a P7 mouse (~ 4 g) after 20 min SIMPLE (MP dose of 50 μg/g). The pup was sacrificed 17 h later (left panel). The infarct area is highlighted with a dashed line. (a’) High-magnification inset from (b) showing MP aggregation in the dMCA (middle panel). Severe brain infarction in the right hemisphere at 3 d after 20 min SIMPLE in a P7 mouse (right panel). MP aggregation was still observed 3 d after 20 min SIMPLE (arrowhead). b, c. Tissue sections from the brain shown in (b, right panel) were stained with FJC. The degenerating neurons are indicated with the white arrowheads in b’, an inset of (c). The distribution of the fluorescence between the margins and center of the field is uneven with our stereoscope, which sometimes leads to a darker halo in the middle of the imaged field. d. Tissue sections from the brain shown in (b, right panel) were stained with Hoechst33342 to label nuclei. Right, ischemic side; left, contralateral side. c’ and d’, high-magnification images from non-infarct and infarct brain regions, respectively. Shrunken nuclei are indicated with arrows in d’

Back to article page