Fig. 2

VGlut-Zal1 drives reporter expression with similar fidelity to VGlut-Gal4 and generates distinct intersected expression pattern in combination of Gal4 lines. In adult fly brains, widespread GFP expression was observed for both VGlut drivers and their combination. a–b Maximum intensity projection images of (a) the brain anterior to the ellipsoid body and (b) the ellipsoid-posterior brain of a UASZ-mCD8GFP/+; VGlut-Zal1/+ fly stained with α-GFP (green) and α-DLG (magenta) antibodies. c–d Maximum intensity projections of the anterior (c) and posterior (d) expression patterns in a UASG-mCD8GFP/+; VGlut-Gal4/+ brain stained with α-GFP (green) and α-DLG (magenta) antibodies. e–f Projection images of an anterior (e) and posterior (f) portions of a UASGZ-mCD8GFP/+; VGlut-Zal1/VGlut-Gal4 brain stained with α-GFP (green) and α-DLG (magenta) antibodies. g–h. VGlut-Gal4 and VGlut-Zal1 are not individually active at non-cognate UAS sites. VGlut-Gal4; UASZ-GFP and VGlut-Zal1; UASG-GFP brains stained with α-GFP showed little or no green fluorescence. i.VGlut-Gal4; UASGZ-GFP and j. VGlut-Zal1; UASGZ-GFP brains were α-GFP-negative. k. Expression pattern of Orco-Gal4 crossed with UASG-GFP. l Intersectional expression pattern of Orco-Gal4 generated using VGlut-Zal1; no GFP expression was observed. m The expression pattern of OK107-Gal4 crossed with UASG-GFP. n The intersectional expression pattern of OK107-Gal4 with VGlut-Zal1. Images show staining with α-GFP (green) and α-DLG (magenta). Scale bars represents 200 μm; dorsal is up