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Fig. 4 | Molecular Brain

Fig. 4

From: A zinc-finger fusion protein refines Gal4-defined neural circuits

Fig. 4

Quantification of Gal4- and Zal1-mediated genetic intersection in Crz cells. A Venn plot shows cell counts of α-GFP and α-Crz antibody staining as percentages. Bar heights are quantitative; bar areas are not. Counts of cells staining positively for Crz were defined as constituting 100% of α-Crz + cells (magenta bar). Counts of cells staining α-GFP+ were defined as the driver’s expression range (green bar). The overlap between α-Crz + and α-GFP+ cells is displayed in white. Left The Crz-Gal4 driver expresses GFP in all seven Crz + neurons, along with expression in 15 ectopic cells. Center Crz-Zal1 expresses in all 7 corazonergic cells along with ectopic expression in less than one cell. Right The Crz-Gal4/Crz-Zal1 double driver expresses in Crz + cells exclusively

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