Fig. 5
From: A zinc-finger fusion protein refines Gal4-defined neural circuits
The Trh-Zal1 + Trh-Gal4 combination drives expression in the majority of serotonergic cells a–d. MIPs of (a) of a UASG-mCD8GFP/+; Trh-Gal4/+ brain stained with α-GFP (green) and α-DLG (magenta) antibodies. b A Trh-Gal4/UASG-mCD8GFP brain stained with α-GFP (green), (c) with α-5HT antibodies and (d) combined image. e-h. MIPs of (e) a UASZ-mCD8GFP/+; Trh-Zal1/+ brain stained with α-GFP and α-DLG (magenta); (f) a Trh-Zal1/UASZ-mCD8GFP brain stained with α-GFP (green), (g) with α-5HT, and (h) combined image. i–l. MIPs of (i) a UASGZ-mCD8GFP/+; Trh-Zal1/Trh-Gal4 brain stained with α-GFP and α-DLG (magenta); (j) a Trh-Zal1; Trh-Gal4/UASGZ-mCD8GFP brain stained with α-GFP, (k) with α-5HT and (l) combined image. m A Trh-Gal4; UASZ-GFP brain stained with α-GFP showed no green fluorescence n. ATrh-Zal1; UASG-GFP brain showed no α-GFP fluorescence. o A Trh-Gal4; UASGZ-GFP brain showed no α-GFP fluorescence p. A Trh-Zal1; UASGZ-GFP brain showed weak GFP expression in a few cells; arrows indicate expression. The brains M–P were stained with α-GFP and α-5-HT. Scale bar represents 200 μm; dorsal is up