Fig. 6
From: A zinc-finger fusion protein refines Gal4-defined neural circuits
Genetic intersection of Trh-Zal1 with Trh-Gal4 and enhancer trap lines results in high-fidelity expression. a. A Venn plot displays α-GFP+ expression as a percentage of α-5-HT+ cells. Left Trh-Gal4 drives expression in 90% of serotonergic neurons, along with 17% of expression in ectopic cells; Center similarly, Trh-Zal1 drives expression in ~ 88% of serotonergic cells with ectopic expression in 4% of 5-HT+ cells. Right The Trh-Gal4/Trh-Zal1 combination drives expression in ~ 82% of serotonergic cells with no expression in ectopic cells. The total-count mean of 5-HT+ cells ranged from 30 to 34 per brain hemisphere. b. The R22H10-Gal4 + Trh-Zal1 combination has 51% extensiveness within the antibody stain, with 75% fidelity. The R53C03-Gal4 + Trh-Zal1 combination: 59% extensiveness and 71% fidelity. R70A11-Gal4 + Trh-Zal1 combination: 49.5% extensiveness and 91% fidelity. R89A09-Gal4 + Trh-Zal1 combination: 47.5% extensiveness and 69.5% fidelity. The total-count mean of 5-HT+ cells ranged from 35 to 42 per brain hemisphere