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Fig. 1 | Molecular Brain

Fig. 1

From: Fibronectin type III domain-containing protein 5 interacts with APP and decreases amyloid β production in Alzheimer’s disease

Fig. 1

Full length of APP and FNDC5 interacted mainly at the specific domain of Aβ sequence. FNDC5 is identified by the red arrow in (a) and the number beside each blot is the protein standard size. In a, the expression of FNDC5 is identified. The left blot was blotted by anti-HA antibody and indicates the overexpressed FNDC5 tagged with HA. The right image was blotted by anti-FNDC5 antibody. The immunoprecipitation was performed in HEK293 cells transfected with APP770 tagged with V5 and FNDC5 tagged with HA. b shows that full length APP associates with FNDC5 in HEK293 cells. Full length APP was detected by anti-V5 antibody, and FNDC5 was detected by anti-HA antibody. c shows that the interaction of endogenous APP and FNDC5 was observed physiologically using the hippocampus of wild type rat (8-weeks old). The blot of 4G8 indicates APP full length

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