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Fig. 5 | Molecular Brain

Fig. 5

From: Fibronectin type III domain-containing protein 5 interacts with APP and decreases amyloid β production in Alzheimer’s disease

Fig. 5

FNDC5 decreased Aβ level secreted into media in HEK293 cells. a indicates the results of Aβ40 and Aβ42 measurements. To compare the secretion of Aβ, the secretion level of APP770-V5 + pcDNA3.1 is referred to as 100%. In addition, we compared the ratio of Aβ42/40, shown in the lower panel; the ratio of APP770-V5 + pcDNA3.1 referred to as 1. The gray and red bar indicate the groups of APP770-V5 + pcDNA3.1 and APP770-V5 + FNDC5-HA, respectively. b shows the alteration of Aβ by the inhibition of Aβ1–16 peptide treatment in HEK293 cells transfected with APP770-V5 and FNDC5-HA. The secretion level of Aβ without the peptide treatment (shown as Aβ1–16 (−)) is referred to as 100%. The lower panel shows the ratio of secreted Aβ42/40 with or without the peptide treatment, where the ratio in the group of Aβ1–16 (−) is referred to as 1. The blots of full length APP and APP C-terminal fragments (CTF) with the expression of FNDC5 were shown in c, and their quantified results were shown in (d). The intensity of APP full length, C99 and C83 are corrected by the level of β-actin. Each band intensity in the group of APP770-V5 + pcDNA3.1 is referred to as 100%. e shows the alteration for sAPPβ and sAPPα in the conditioned media when APP770-V5 and FNDC5-HA was transiently transfected. The group of APP770-V5 + pcDNA3.1 is referred to as 100%. In HEK293 cells transfected with C99-myc and FNDC5-HA, a significant but less drastic decrease of Aβ in the media was shown in (f). The group of C99-myc + pcDNA3.1 is referred to as 100%. To compare the ratio of Aβ42/40, the ratio in the group of C99-myc + pcDNA3.1 is referred to as 1. As compared to the results of a, FNDC5 led to greater Aβ reduction in the cells with full length APP than with C99. The significances of p-value < 0.05 and < 0.01 were indicated as *, ** respectively

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