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Fig. 6 | Molecular Brain

Fig. 6

From: 5-HTR2A and 5-HTR3A but not 5-HTR1A antagonism impairs the cross-modal reactivation of deprived visual cortex in adulthood

Fig. 6

5-HTR antagonist treatment from week 5–7 post-ME affects neuronal activity levels in S1BF. a-d Images of 3 adjacent sections (− 1.7-(− 1.9); 2.5–4; 1, relative to Bregma) from adult 7wME mice, injected during the last 3 weeks of the 7wME recovery period with (a) saline, (b) 5-HTR1A antagonist WAY-100635, (c) 5-HTR2A antagonist ketanserin, (d) 5-HTR3A antagonist ondansetron. Corresponding pseudocolor representations are displayed next to their respective ISH sections. e Line graphs illustrating the relative zif268-mRNA expression level, measured as the average OD-value/segment, for saline-injected 7wME mice. For supra- and granular layers II/III-IV (e, left panel, dark gray line) and for infragranular layers V-VI (e, right panel, light gray line) the expression levels are displayed along the 3 predefined somatosensory subdivisions (black arrowheads are in accordance with the small arrowheads in a-d). Error bars represent the SEM of the mean OD value in each segment. Relative zif268-mRNA expression levels are shown as OD-values averaged over the primary somatosensory barrel cortex (S1BF) for supra- and granular layers (f-h, left panel) and infragranular layers (f-h, right panel). Comparison of the OD-values of saline-injected (light gray bars) and WAY-100635-injected 7wME mice (orange bars) indicates no post-ME changes in neuronal activity in S1BF due to long-term i.p. injection of 5-HTR1A antagonist (f). Comparison of the OD-values of saline-injected (light-gray bars) and ketanserin-treated 7wME mice (green bars) indicates increased neuronal activity across all layers of the S1BF (g). Comparison of the OD-values of saline-injected (light-gray bars) and ondansetron-treated 7wME mice (blue bars) indicates increased neuronal activity across all layers of the S1BF (h). The number of animals (n) is represented in the bars. *P < 0.05

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