Fig. 1

High-fat diet (HFD) fed mice gained weight and presented hyperglycemia. (a) Experimental schedule. (b) Body weight was measured every 4 weeks on each diet group. (c) Fasting serum glucose level. (d) Glucose tolerance test. (e) Insulin tolerance test. (f) Serum insulin level measured using ELISA in the 24th week of the diet (p = 0.074). The p-values were obtained from the two-tailed Student’s t-test between HFD-fed mice and control groups (n = 10 for both groups) *** p < 0.001. Abbreviation: BW = body weight; GL = serum glucose level; NOR = novel object recognition test; MWM = water maze behavior test; ¹³C MRS = ¹³C magnetic resonance spectroscopy; ¹³C CSI = ¹³C chemical shift image; DCE-MRI = dynamic contrast-enhanced magnetic resonance imaging