Fig. 5

Increased lactate production and decreased pyruvate dehydrogenase (PDH) activity in HFD fed mice. (a) Amount of lactate in 40 μg of a lysate of cortex, hippocampus and striatum tissues (n = 5 for both group). (b) Serum lactate level measured using ELISA (10.64 ± 1.745 vs. 13.02 ± 0.75; n = 10 each). (c) PDH activity measured in the cortex, hippocampus and striatum tissues (n = 5–6 for both groups). (d) Quantified LDHA by the ratio to β-actin and cropped images (n = 5 for both groups). (e) Quantified LDHB by the ratio to β-actin and cropped images (n = 5 for both groups). (f) Quantified Phosphorylated PDH by the ratio to total PDH and cropped images (n = 5 for both groups). * p < 0.05, ** p < 0.01, *** p < 0.001