Fig. 5
From: Pathological changes of distal motor neurons after complete spinal cord injury
Gene expression of lumbar motor neurons reveal decreased neuronal activity in the chronic phase of SCI. A: The mRNA expression of neuronal activity markers c-fos and CaMK2a in lumbar motor neurons isolated by LMD from the Naive and Tx3m groups (n = 8 mice per group). B: Immunohistochemistry for the neuronal activity markers c-fos (red) and NeuN (green) in the lumbar motor neurons, with nuclear counterstain in Hoechst (blue). The lower right images are magnifications of the boxed areas. *P < 0.05, Wilcoxon rank sum test (A). Data are presented as the mean ± SEM. Scale bars: 100 μm (B); 10 μm (B-a and B-b in the boxed areas)