Fig. 3
From: Hyperexcitability of the local cortical circuit in mouse models of tuberous sclerosis complex
Electrophysiological recordings from layer 2/3 pyramidal neurons in P28–30 control and Syn1-Cre x Tsc1fl/fl mouse visual cortex. a-b Sample traces of AMPAR-mEPSC recorded at − 40 mV and of GABAR-mIPSC recorded at 0 mV from same pyramidal neurons in control (blue traces, Tsc1fl/fl mouse without Cre) and Syn1-Cre x Tsc1fl/fl (red traces) mice. Scale bar: 20 pA, 200 ms. c-d Pooled data showing no significant differences in averaged AMPAR-mEPSC amplitude (c, Control, 5.73 ± 0.15 pA vs. Syn1-Cre x Tsc1fl/fl, 5.88 ± 0.32 pA, P = 0.79) and frequency (d, Control, 2.55 ± 0.32 Hz vs. Syn1-Cre x Tsc1fl/fl, 3.91 ± 0.79 Hz, P = 0.13) between the mutant and control animals. e Top, averaged AMPAR-mEPSCs obtained from control (blue, shown in a) and Syn1-Cre x Tsc1fl/fl (red, shown in b) mice, bottom, normalization and superposition of the above averaged AMPAR-mEPSCs. Scale bars: 2 pA, 4 ms. f-g Pooled data showing no significant differences in averaged AMPAR-mEPSC rise time (f, Control, 1.07 ± 0.06 ms vs. Syn1-Cre x Tsc1fl/fl, 1.09 ± 0.11 ms, P = 0.95) and decay time (g, Control, 4.77 ± 0.31 ms vs. Syn1-Cre x Tsc1fl/fl, 5.28 ± 0.62 ms, P = 0.56) between the mutant and control animals. h-i Pooled data showing no significant differences in averaged GABAR-mIPSC amplitude (H, Control, 9.77 ± 0.6 pA vs. Syn1-Cre x Tsc1fl/fl, 9.71 ± 0.55 pA, P = 0.95) and significant difference in averaged GABAR-mIPSC frequency (I, Control, 7.0 ± 0.38 Hz vs. Syn1-Cre x Tsc1fl/fl, 4.5 ± 0.55 Hz, P = 0.002) between the mutant and control animals. ** P < 0.01. j Top, averaged GABAR-mIPSCs obtained from control (blue, shown in A) and Syn1-Cre x Tsc1fl/fl (red, shown in B) mice, bottom, normalization and superposition of the above averaged GABAR-mIPSCs. Scale bars: 4 pA, 20 ms. k-l Pooled data showing no significant differences in averaged GABAR-mIPSC rise time (K, Control, 1.67 ± 0.13 ms vs. Syn1-Cre x Tsc1fl/fl, 1.57 ± 0.14 ms, P = 0.63) and decay time (L, Control, 19.8 ± 2.14 ms vs. Syn1-Cre x Tsc1fl/fl, 17.17 ± 1.62 ms, P = 0.48) between the mutant and control animals. For pooled data, unpaired two-tailed t-test was used, the open diamonds represent individual experiments and the filled diamonds are means of the experiments in groups, n = 10/3 for control and n = 13/3 for Syn1-Cre x Tsc1fl/fl groups