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Fig. 5 | Molecular Brain

Fig. 5

From: Hyperexcitability of the local cortical circuit in mouse models of tuberous sclerosis complex

Fig. 5

Electrophysiological recordings from layer 2/3 pyramidal neurons in P29 control and Pv-Cre x Tsc1fl/fl mouse visual cortex. a-b Sample traces of AMPAR-mEPSC recorded at − 40 mV and of GABAR-mIPSC recorded at 0 mV from same pyramidal neurons in control (blue traces, Tsc1fl/fl mouse without Cre) and Pv-Cre x Tsc1fl/fl (red traces) mice. Scale bar: 20 pA, 200 ms. (C-D) Pooled data showing no significant differences in averaged AMPAR-mEPSC amplitude (C, Control, 6.69 ± 0.3 pA vs. Pv-Cre x Tsc1fl/fl, 6.13 ± 0.17 pA, P = 0.12) and frequency (D, Control, 1.79 ± 0.2 Hz vs. Pv-Cre x Tsc1fl/fl, 1.74 ± 0.3 Hz, P = 0.88) between the mutant and control animals. e Top, averaged AMPAR-mEPSCs obtained from control (blue, shown in a) and Pv-Cre x Tsc1fl/fl (red, shown in b) mice, bottom, normalization and superposition of the above averaged AMPAR-mEPSCs. Scale bars: 2 pA, 4 ms. f-g Pooled data showing no significant differences in averaged AMPAR-mEPSC rise time (F, Control, 1.07 ± 0.06 ms vs. Pv-Cre x Tsc1fl/fl, 1.27 ± 0.07 ms, P = 0.06) and decay time (G, Control, 5.15 ± 0.29 ms vs. Pv-Cre x Tsc1fl/fl, 5.88 ± 0.23 ms, P = 0.07) between the mutant and control animals. h-i Pooled data showing no significant differences in averaged GABAR-mIPSC amplitude (H, Control, 8.08 ± 0.19 pA vs. Pv-Cre x Tsc1fl/fl, 8.05 ± 0.28 pA, P = 0.93) and frequency (I, Control, 2.65 ± 0.31 Hz vs. Pv-Cre x Tsc1fl/fl, 3.86 ± 0.71 Hz, P = 0.14) between the mutant and control animals. j Top, averaged GABAR-mIPSCs obtained from control (blue, shown in A) and Pv-Cre x Tsc1fl/fl (red, shown in B) mice, bottom, normalization and superposition of the above averaged GABAR-mIPSCs. Scale bars: 4 pA, 20 ms. k-l) Pooled data showing no significant difference in averaged GABAR-mIPSC rise time (K, Control, 2.22 ± 0.18 ms vs. Pv-Cre x Tsc1fl/fl, 2.35 ± 0.15 ms, P = 0.59) and significant difference in averaged GABAR-mIPSC decay time (L, Control, 15.4 ± 0.78 ms vs. Pv-Cre x Tsc1fl/fl, 18.1 ± 0.93 ms, P = 0.04) between the mutant and control animals. For pooled data, unpaired two-tailed t-test was used, the open diamonds represent individual cells and the filled diamonds are means of the experiments in groups, n = 8/2 for control and n = 9/2 for Pv-Cre x Tsc1fl/fl groups

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