Fig. 6
From: Hyperexcitability of the local cortical circuit in mouse models of tuberous sclerosis complex
Electrophysiological recordings from layer 2/3 pyramidal neurons in P29 control and Sst-Cre x Tsc1fl/fl mouse visual cortex. a-b Sample traces of AMPAR-mEPSC recorded at − 40 mV and of GABAR-mIPSC recorded at 0 mV from same pyramidal neurons in control (blue traces, Tsc1fl/fl mouse without Cre) and Sst-Cre x Tsc1fl/fl (red traces) mice. Scale bar: 20 pA, 200 ms. c-d Pooled data showing no significant differences in averaged AMPAR-mEPSC amplitude (c, Control, 6.93 ± 0.39 pA vs. Sst-Cre x Tsc1fl/fl, 7.69 ± 0.58 pA, P = 0.28) and frequency (d, Control, 1.59 ± 0.18 Hz vs. Sst-Cre x Tsc1fl/fl, 1.77 ± 0.31 Hz, P = 0.66) between the mutant and control animals. e Top, averaged AMPAR-mEPSCs obtained from control (blue, shown in a) and Sst-Cre x Tsc1fl/fl (red, shown in b) mice, bottom, normalization and superposition of the above averaged AMPAR-mEPSCs. Scale bars: 2 pA, 4 ms. f-g Pooled data showing no significant differences in averaged AMPAR-mEPSC rise time (F, Control, 1.15 ± 0.1 ms vs. Sst-Cre x Tsc1fl/fl, 1.11 ± 0.14 ms, P = 0.82) and decay time (G, Control, 5.94 ± 0.49 ms vs. Sst-Cre x Tsc1fl/fl, 5.71 ± 0.54 ms, P = 0.74) between the mutant and control animals. h-i Pooled data showing no significant differences in averaged GABAR-mIPSC amplitude (H, Control, 11.2 ± 0.42 pA vs. Sst-Cre x Tsc1fl/fl, 11.5 ± 0.77 pA, P = 0.74) and frequency (I, Control, 3.26 ± 0.37 Hz vs. Sst-Cre x Tsc1fl/fl, 3.29 ± 0.55 Hz, P = 0.97) between the mutant and control animals. j Top, averaged GABAR-mIPSCs obtained from control (blue, shown in A) and Sst-Cre x Tsc1fl/fl (red, shown in B) mice, bottom, normalization and superposition of the above averaged GABAR-mIPSCs. Scale bars: 4 pA, 20 ms. k-l Pooled data showing no significant difference in averaged GABAR-mIPSC rise time (K, Control, 1.83 ± 0.2 ms vs. Sst-Cre x Tsc1fl/fl, 1.75 ± 0.21 ms, P = 0.79) and decay time (L, Control, 14.45 ± 0.66 ms vs. Sst-Cre x Tsc1fl/fl, 14.16 ± 1.45 ms, P = 0.85) between the mutant and control animals. For pooled data, unpaired two-tailed t-test was used, the open diamonds represent individual cells and the filled diamonds are means of the experiments in groups, n = 7/1 for control and n = 9/2 for Sst-Cre x Tsc1fl/fl groups