Fig. 2

Impaired motor skills in mice lacking the cerebellothalamic tracts. a Intact spontaneous overground locomotion. Distance traveled, velocity, or the number of entries into the center zone of the open field are not affected by the IT treatment. n = 8 mice for the PBS-treated group and n = 6 mice for the IT-treated group. b Parameters measured in the gait analysis. c Representative walking footprint patterns of each mouse. No obvious difference between the groups is observed. d Quantitative analyses of the footprints. Stride length of each limb, base width of front limbs or hind limbs, or paw overlap of left limbs or right limbs are not altered by the IT treatment. n = 8 mice for the PBS-treated group and n = 6 mice for the IT-treated group. e Impaired motor coordination in mice lacking the cerebellothalamic tracts. Mice treated with the IT solution show shorter latency until fall in the rotarod test. n = 12 mice for the PBS-treated group and n = 9 mice for the IT-treated group. **p < 0.01 (two-way ANOVA). f Impaired forelimb reaching in mice lacking the cerebellothalamic tracts. The success rate in the single-seed reaching test is decreased in mice treated with the IT solution. n = 8 mice for the PBS-treated group and n = 6 mice for the IT-treated group. *p < 0.05 (Student’s t-test) g Intact correct response rate in the place discrimination test. The correct response rate is not affected in either learning phase by the IT treatment. n = 12 mice for the PBS-treated group and n = 9 mice for the IT-treated group. h Similar escape latency in the Morris water maze test. The latency to find the platform is not affected in either learning phase by the IT treatment. n = 8 mice for each group. i Normal correct response rate in the T-maze test. The correct response rate is not affected in either learning phase by the IT treatment. n = 10 mice for the PBS-treated group and n = 9 mice for the IT-treated group. All data are expressed as the mean ± s.e.m. Scale bar: (c) 10 mm