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Fig. 3 | Molecular Brain

Fig. 3

From: Motor skills mediated through cerebellothalamic tracts projecting to the central lateral nucleus

Fig. 3

Axonal arborization of the DN neurons innervating the CL to the VA/VL. a Selective targeting of axonal arborization of the DN neurons innervating the CL. Injection of the NeuRet lentiviral vector encoding Cre into the CL induced the expression of Cre in various brain regions innervating the CL, including the DN. Subsequent injection of the AAV2-FLEX-EGFP into the DN selectively labels axonal terminals branched from the DN-CL tracts. b GFP expression in the DN. The GFP expression is selectively observed in the DN of mice injected with the NeuRet-Cre vector. c GFP expression in the VA/VL and the CL. The GFP expression is observed in the rostral VA/VL and the CL of mice injected with the NeuRet-Cre vector. d GFP expression in the RN. Only a small number of GFP expression is observed in the RN of mice injected with the vector. Scale bars: (b) 200 μm, (c) 400 μm for lower magnification and 100 μm for higher magnification, and (d) 400 μm. Abbreviations: Aq, aquedact; D3V, dorsal third ventricle; DC, dorsal cochlear nucleus; DG, dentate gyrus; fr, fasciculus retroflexus; Hb, habenular; ic, internal capsule; ml, medial lemniscus; PAG, periaqueductal gray

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