Fig. 1

Western blots showing Cav3.2 and ENaC complexes in neuronal tissue. Cav3.2 immunoprecipitates from mouse whole brain lysates were probed for either (a) α-, n = 3 (b) β-, n = 3 (c) γ-ENaC subunits, n = 3 (arrows indicate full-length and cleaved proteins) or (d) Cav3.2 calcium channels, n = 4. Actin loading controls are shown. (e) Cav3.2 immunoprecipitates from dorsal horn were probed for either β-ENaC, n = 3 (IgG lane is cut) or (f) γ-ENaC subunits, n = 3 (arrows indicate full-length and cleaved proteins). α-Tubulin loading controls are shown. (g) Cav3.2 immunoprecipitates from lumbar dorsal root ganglia (L3-L6) were probed for either β-ENaC, n = 3 or (h) γ-ENaC subunits, n = 3 (arrows indicate full-length and cleaved proteins). α-Tubulin loading controls are shown