Fig. 5

αβγ-ENaC mediated effects on Cav3.2 surface pool and calcium currents. (a) αβγ-ENaC increases the Cav3.2 surface pool in tsA201. Cells were labelled with biotin and transfected with either Cav3.2 alone or Cav3.2 + α-, β-, or γ- ENaC subunits individually or Cav3.2 + αβγ-ENaC fully assembled channels, n = 3–4. An α-tubulin loading control is shown. (b) Quantification analysis of Cav3.2 surface pool. Statistical analysis was performed with ANOVA. (c) αβγ-ENaC significantly increases Cav3.2 peak current density. Average current densities (pA/pF) as a function of voltage in CAD cells transfected with empty plasmid (open squares) or αβγ-ENaC (black and white squares). Inset: voltage for half activation. (d) Peak current density measured at − 25 mV in absence or presence of αβγ-ENaC. Cells were incubated with 100 nM amiloride which was washed off before experimentation