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Fig. 3 | Molecular Brain

Fig. 3

From: LAMP5 in presynaptic inhibitory terminals in the hindbrain and spinal cord: a role in startle response and auditory processing

Fig. 3

LAMP5 is localized in the inhibitory synaptic terminals. a LAMP5 and VIAAT expression in the adult brainstem. Lower panels: higher-magnification images of the superior olivary complex (SOC) at the positions indicated in the top images (rectangles with white dashed lines). Scale bars: upper, 500 μm; lower, 100 μm. b LAMP5 and VIAAT expression in the cervical spinal cord. Scale bars, 200 μm. c and d Confocal images of the lateral superior olive (LSO) and ventral cochlear nucleus (VCO) regions (c) and the ventral horn of the cervical spinal cord (d). LAMP5 was double-stained with VGluT1, VGluT2 or VIAAT. Scale bars, 5 μm. e Schematic diagram of the SOC nuclei. The medial nucleus of the trapezoid body (MNTB) neurons send inhibitory projection to the LSO, medial superior olive (MSO) and superior paraolivary nucleus (PSV) neurons. f Distribution of Lamp5-expressing neurons in the SOC. In situ hybridization data is from the Allen Mouse Brain Atlas (Experiment No. 70927827) [23]. g LAMP5 expression in MNTB neurons. The cell body of an MNTB neuron can be identified because excitatory synaptic terminals surround it. Right panel: Higher-magnification image of the MNTB neurons indicated by the white square in the left image. Scale bars: left, 25 μm; right, 10 μm

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