Fig. 4

Generation of a Lamp5 KO mouse. a Schematic diagram of the wild-type (WT) and Lamp5 KO alleles. Arrows, CRISPR/Cas9 target sites; gray box, untranslated region; black box, coding region; solid line, intron; dashed line, deleted region in the KO allele. Exon 2 is fused with exon 6. b DNA sequences of the WT and KO alleles. The mutated sequence is shown in red. A termination codon appears at the beginning of exon 6 (underlined). c Amino acid sequences of proteins encoded by the WT and KO alleles. The epitope used for primary antibody production is underlined. d LAMP5 protein expression in whole brain lysates of WT (+/+), heterozygous and homozygous Lamp5 KO (+/− and −/−) mice. β-actin served as a loading control