Fig. 6
Behavioral analysis of Lamp5 KO mice. a Open field test. There was no significant difference between genotypes in the total distance travelled or in the time spent in the outer or inner zones (two-tailed Welch’s t-test, n = 8 mice for each genotype). b Elevated plus maze test. There was no significant difference between genotypes in time spent in the open arm or the closed arm (two-tailed Welch’s t-test, n = 8 mice for each genotype). c Rotarod test. Two-way repeated-measures ANOVA (genotype × time) revealed a significant main effect of time (F(3,42) = 9.458, p < 0.0001), a non significant effect of genotype (F(1,15) = 2.51, p > 0.05) and a non significant interaction between factors (F(3,42) = 0.1307, p = 0.9413). Body weight after the fourth trial was not different between genotypes (two-tailed Welch’s t-test, n = 8 mice for each genotype). d Startle response to 120 dB white noise stimuli. The response was larger in KO mice (p < 0.05, Two-tailed Welch’s t-test, n = 5 mice for each genotype). e Percent inhibition of auditory startle response by prepulse stimulation. Two-way repeated-measures ANOVA (genotype × prepulse) revealed a significant effect of prepulse intensity (F(3,32) = 23.0, p < 10− 7) and of genotype (F(1,32) = 13.2, p < 0.001) but no significant interaction between factors (F(3,32) = 2.90, p > 0.05). f Startle response to air-puff stimuli. The response was larger in KO mice (p < 0.05, Two-tailed Welch’s t-test, n = 8 mice for each genotype). * p < 0.05, *** p < 0.001. Error bars indicate SEM