Fig. 3
From: Sacs R272C missense homozygous mice develop an ataxia phenotype
Progressive Purkinje cell loss in SacsR272C mice. (a, b) Nissl stain of 45 day-old vermal cerebellar sections show normal cerebellar structure and lobulation in SacsR272C mice (b) compared to control (a). (c-d) Calbindin immunolabeling on vermal sagittal brain sections from 300 day-old mice. SacsR272C mice display cerebellar PC loss (d1, yellow bracket) compared to age-matched controls (c1). (e and f) Neuronal cell counts in the anterior (I to VI) and posterior (VII to X) lobules at different ages, demonstrate significant loss of Purkinje cells in SacsR272C mice starting at 90 days of age in the anterior lobules (e). Some neuronal cell loss is observed in the posterior lobules at 365 days (f). Data represent means ± SEM, number of mice per group is indicated in each bars. ***P < 0.001, *P < 0.05 (two-way ANOVA with repeated-measures followed by Tukey’s post hoc comparison). Scale bar in d1 = 100 μm