Fig. 2
From: Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
Effect of diazepam (DZP) on anxiety measurements during CS presentation. a Experimental procedures. Mice were divided to two groups with comparable freezing levels after fear retention test. They were tested on the EPM (saline, n = 12; DZP, n = 15) or in the shuttle box (saline, n = 8; DZP, n = 8) 48 h later, and received either DZP or Sal injection 30 min before the test (i.p; 1.5 mg/kg). Two days later, the shuttle box group was tested for fear retention (DZP, n = 8; saline, n = 8) with DZP or Sal injected 30 min before testing. b Open arm time on the EPM during CS presentation in both diazepam and saline group. c Number of open arm entries on the EPM during CS presentation in both diazepam and saline group. d Light time (%) in the shuttle box during CS presentation in both diazepam and saline group. e Freezing levels during fear recall in context B in both diazepam and saline group. *, p < 0.05; ***, p < 0.001; ##, p < 0.01; ###, p < 0.001. *, comparing CS to BL; #, comparing Sal to DZP. Data were analyzed by Two-way RM ANOVA with Bonferroni post-test