Fig. 5
From: Conditioned stimulus presentations alter anxiety level in fear-conditioned mice
Calcium responses in vCA1 neurons that projecting to lateral hypothalamus during CS presentation. a Sample image of transfected neurons in vCA1. Scale bars, 100 μm. b Sample Ca2+ response showing elevation in vCA1 neurons during exploration of open arms on the EPM. Scale bars, 10% ΔF/F; 10 s. c Experimental procedures. d Averaged population Ca2+ responses showed gradual reduction during CS presentations which gradually recovered towards baseline after CS termination. e Quantification of changes in Ca2+ responses from data in (d) with integrated area from the last 10 s during CS presentations. f Delta integrated areas from the last 10 s during CS presentations after injection of either Dzp or Sal. Delta integrated area = integrated area after injection - integrated area before injection. *, p < 0.05