Fig. 1
From: ZnT3 expression levels are down-regulated in the brain of Mcoln1 knockout mice
Baseline Slc30a3 mRNA and Slc30a3 (ZnT3) protein expression levels. a Transcriptomic analysis of brain tissues from Mcoln1−/− KO mice (KO1-KO3, n = 3) and Mcoln1+/+ WT (WT1-WT3, n = 3) littermate control mice. b Real-time qPCR analysis of relative Slc30a3 mRNA expression levels from brain tissues taken from Mcoln1−/− KO and Mcoln1+/+ WT littermate control mice. The qPCR experiments were done in triplicate wells, normalized using 18S rRNA, and analyzed using the Standard Curve method. The data are represented as mean ± SD (*p < 0.05, Student’s t-test, paired, n ≥ 3). c Integrated density value analysis of Slc30a3 (ZnT3) protein bands normalized with beta-Actin bands from two independent Western blot experiments. The relative Slc30a3 protein expression levels in Mcoln1−/− KO-A and KO-B mouse brains are significantly reduced in comparison to Mcoln1+/+ WT-A mouse brain (**p < 0.01, Student’s t-test, paired, n ≥ 2). d Representative Western blot image of ZnT3 (green; monomer: ~ 42 kDa; dimer: ~ 84 kDa) and beta-Actin proteins (red; ~ 41 kDa). Each lane corresponds to: L, protein ladder; 1, Mcoln1+/+ WT-A control brain; 2, Mcoln1+/− heterozygote control brain; 3, Mcoln1−/− KO-A brain; and 4, Mcoln1−/− KO-B brain. The blot was probed with anti-beta-Actin mouse monoclonal antibody and anti-ZnT3 rabbit polyclonal antibody, and imaged using LICOR Odyssey Sa infrared scanner at 700 nm and 800 nm channels, respectively