Fig. 3From: Prenatal selective serotonin reuptake inhibitor (SSRI) exposure induces working memory and social recognition deficits by disrupting inhibitory synaptic networks in male miceIncreased excitability and serotonergic modulation of FS interneurons in PrL of FLX-treated mice. (a) Schematic diagram of the mouse PFC as outlined by the red dashed lines. The black box indicates a high magnification view of the neurons that were patched. We performed whole-cell patch-clamp recordings of putative fast-spiking (FS) interneurons in L5 of the PrL. (b) Representative image of a biocytin-filled FS interneuron in L5. Inset – co-staining for biocytin and parvalbumin, a marker of FS interneurons. (c) Characteristic responses of FS interneurons from SAL-treated mice to current injections (− 450 pA, 200 pA, and 450 pA) showing low adaptation to repeated firing. (d) Representative traces of FS interneurons from SAL-treated mice showing responses to current injections (200 pA) at baseline (CTRL), during the bath application of 5-HT (5-HT), and the bath application of the 5-HT2AR antagonist MDL. (e) Bar plot summarizing the effects of the 5-HT and MDL treatment on FS interneurons in SAL-treated mice (f) Representative traces of FS interneurons from FLX-treated mice show the responses to current injections (200 pA) under CTRL, 5-HT and MDL conditions. (g) Bar plot summarizing the effects of the 5-HT and MDL treatments on FS interneurons in FLX-treated mice. Data are presented as means ± SEM. All data were analyzed using the Wilcoxon signed ranks test. **p < 0.01Back to article page