Fig. 2

Segregated expressions of Cdh9 and Cdh11 in ASD-associated cerebellar areas. In situ hybridization of Cdh9 and Cdh11 were performed on adjacent sagittal sections (P4) at different levels. a-c sections show in situ hybridization signals at level S10, and d-f sections show expression signals at level S6. Images were merged in (c) and (f) with signal intensity coded with pseudo colors. In lobules VI/VII of vermis, an ASD-associated area of the cerebellum, Cdh11 was expressed in the central part and Cdh9 was expressed in the surrounding areas, forming a segregated expression pattern. In Crus I (CI) and Crus II (CII) of the hemisphere, another ASD-associated area of the cerebellum, they formed a similar segregated expression pattern with Cdh11 in the central part of Crus I and Cdh9 in the surrounding areas. Scale bar: 500 μm