Fig. 4

Differentiation of hG008 cells. a hG008 cells cultured under serum-free sphere-forming conditions were immunostained with anti-Nestin, anti-GFAP, anti-O4, and anti-NeuN antibodies. Scale bar, 100 μm. b–d Sections from brain slice cultures (after 96 h of culture) obtained from mice implanted with hG008 cells were immunostained with anti-Nestin, anti-GFAP, anti-O4, and anti-NeuN antibodies. White arrows indicate GFAP-positive hG008 cells. Scale bar, 100 μm