Fig. 6

Whole-brain imaging of GSC invasion. Brain tissues from mice 45 days after implantation of hG008 cells were cleared and 3D fluorescence images were reconstructed. See Additional files 7, 8, 9 and 10. a Clearing of a 4-mm thick brain slice. b–e Representative fluorescence images of the striatum (b), cortical area (c), inferior horn of the lateral ventricle (d), and corpus callosum (e) are shown. The imaged area and direction are indicated. Scale bar, 100 μm. f Schematic illustration of GSC invasion in the brain. GSCs invaded along axon bundles from the implanted site toward the contralateral side through the corpus callosum. GSCs also invaded toward the inferior horn of the lateral ventricle. Red arrows indicate the direction of GSC migration