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Fig. 3 | Molecular Brain

Fig. 3

From: SGIP1α functions as a selective endocytic adaptor for the internalization of synaptotagmin 1 at synapses

Fig. 3

a Average traces of Syt1-pHluorin signal in control and SGIP1α KD during pH exchange experiments. Non-permeable pH 5.5 acid solution (Q1 and Q2 period) rapidly quenches all surface Syt1-pHluorin. After 50 s, the solution was changed back to pH 7.4. After 300 APs at 10 Hz, the extracellular solution was changed again to pH 5.5 for 50 s then back to pH 7.4. Net Syt-pH fluorescence changes were normalized to the initial values for individual experiments and averaged. Steady and stimulation represents the surface fraction of Syt1-pHluorin probe before and after stimulation, respectively. b, c Bar graphs indicate the surface fraction of Syt1-pHluorin probe before stimulation (b, control, 0.58 ± 0.03, n = 4; KD, 0.58 ± 0.06, n = 4) and after stimulation (c, control, 0.57 ± 0.03, n = 4; KD, 0.80 ± 0.07, n = 4). Data are presented as mean ± SEM. *p < 0.05

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Molecular Brain

ISSN: 1756-6606