Table 2 Primers used for gene expression analysis of genes associated with microglia activation
From: Absence of BBSome function leads to astrocyte reactivity in the brain
Genes | Primers |
|---|---|
Iba1 | Forward: 5’gcagcacttgggtaacacct3’ Reverse: 5’taaccacccctcctttcctc |
Cd68 | Forward: 5’actggtgtagcctagctggt3’ Reverse: 5’ccttgggctataagcggtcc3’ |
Tlr3 | Forward: 5’ccagaagaatctaatcaaattagatttgtc3’ Reverse: 5’ttttgctaagagcagttcttggag3’ |
Tlr4 | Forward:5’ggcaacttggacctgaggag3’ Reverse:5’catgggctctcggtccatag3’ |
Gapdh | Forward: 5’catttcctggtatgacaatgaatacg3’ Reverse:5’tccagggtttcttactccttgga3’ |
TMEM119 | Forward:5’gcatgaagaaggcctggac3’ Reverse:5’ctgggtagcagccagaatgt3’ |