Table 3 Antibodies used either in western blot analysis or immunofluorescence imaging
From: Absence of BBSome function leads to astrocyte reactivity in the brain
Antibody | Company | Dilution |
|---|---|---|
GFAP | Abcam | 1:5000 western and immunofluorescence |
SERPINA3N | R&D | 1:2000 Western blot |
LCN2 | Abcam | 1:2000 Western blot |
SNAP25 | Proteintech | 1:3000 Western blot |
CD68 | Abcam | 1:1000 Western blot |
COMPLEXIN 2 | Proteintech | 1:2000 Western blot |
PSD95 | Santa Cruz | 1:500 Western blot |
IBA1 | Abcam | 1:1000 western blot and 1:200 Immunofluorescence |
TTC8 | Sigma | 1:500 Western blot |
GAPDH | Thermofisher | 1:30000 western blot |
HOMER | Santa Cruz | 1:500 Western blot |
GLUR1 | Santa Cruz | 1:1000 western blot |
VIMENTIN | Abcam | 1:1000 western and immunofluorescence |
BBS2 | Santa Cruz | 1:200 western blot |
BBS3 | Proteintech | 1:500 western blot |
SYP | Proteintech | 1:1000 western blot |
NEUN | Abcam | 1:1000 western blot and immunofluorescence |
STBPX1 | Proteintech | 1:1000 western blot |
VDAC | Abcam | 1:1000 western blot |
IL1β | Abcam | 1:100 Immunofluorescence |