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Fig. 8 | Molecular Brain

Fig. 8

From: Stimulation induces gradual increases in the thickness and curvature of postsynaptic density of hippocampal CA1 neurons in slice cultures

Fig. 8

Four NMDA-treated (50 μM, 2 min) samples of dissociated hippocampal neuronal cultures were post-fixed differently as indicated. Samples were from sister cultures treated in parallel. The deeper layer of the PSD (marked by asterisks*) stained more prominently with regular osmium tetroxide (1% OsO4; a, c) than with “reduced osmium” (1% OsO4 and 1% potassium ferrocyanide; b, d). This preferential staining by regular osmium persisted with or without 1% tannic acid treatment. Scale bar = 0.1 μm

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Molecular Brain

ISSN: 1756-6606